期刊
LAB ON A CHIP
卷 11, 期 7, 页码 1256-1261出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0lc00613k
关键词
-
类别
资金
- UK Engineering and Physical Sciences Research Council [EP/C54269X/1, EP/G00465X/1]
- Institute of Chemical Biology
- Engineering and Physical Sciences Research Council [EP/G00465X/1, EP/C54269X/1] Funding Source: researchfish
We have developed a generic platform to undertake the analysis of protein copy number from single cells. The approach described here is 'all-optical' whereby single cells are manipulated into separate analysis chambers using an optical trap; single cells are lysed by a shock wave caused by laser-induced microcavitation, and the protein released from a single cell is measured by total internal reflection microscopy as it is bound to micro-printed antibody spots within the device. The platform was tested using GFP transfected cells and the relative precision of the measurement method was determined to be 88%. Single cell measurements were also made on a breast cancer cell line to measure the relative levels of unlabelled human tumour suppressor protein p53 using a chip incorporating an antibody sandwich assay format. These results suggest that this is a viable method for measuring relative protein levels in single cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据