期刊
LAB ON A CHIP
卷 10, 期 10, 页码 1262-1266出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/b927460j
关键词
-
类别
资金
- NEDO Industrial Technology Research Assistance Project
- JSPS Core-to-Core Program
- Knut and Alice Wallenberg's foundation
- Swedish Research Council
- JST
- VINNOVA
- SSF grant Multidisciplinary Bio
The sensitive detection and quantification of DNA targets in the food industry and in environmental and clinical settings are issues of utmost importance in ensuring contamination-free food, monitoring the environment, and battling disease. Selective probes coupled with powerful amplification techniques are therefore of major interest. In this study, we set out to create an integrated microchemical chip that benefits from microfluidic chip technology in terms of sensitivity and a strong detection methodology provided jointly by padlock probes and rolling circle amplification (RCA). Here, we have integrated padlock probes and RCA into a microchip. The chip uses solid phase capture in a microchannel to enable washing cycles and decrease analytical area, and employs on-bead RCA for single-molecule amplification and detection. We investigated the effects of reagent concentration and amount of padlock probes, and demonstrated the feasibility of detecting Salmonella.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据