期刊
LAB ON A CHIP
卷 10, 期 20, 页码 2651-2654出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0lc00005a
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类别
资金
- NRL (National Research Lab)
- Korea Science and Engineering Foundation (KOSEF), Republic of Korea [R0A-2007-000-20086-0]
- Ministry of Education, Science and Technology [2009-0082201]
We have developed a multi-layer, microfluidic array platform containing concave microwells and flat cell culture chambers to culture embryonic stem (ES) cells and regulate uniform-sized embryoid body (EB) formation. The main advantage of this platform was that EBs cultured within the concave microwells of a bottom layer were automatically replated into flat cell culture chambers of a top layer, following inversion of the multi-layer microfluidic array platform. This allowed EB formation and EB replating to be controlled simultaneously inside a single microfluidic device without pipette-based manual cell retrieval, a drawback of previous EB culture methods.
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