期刊
LAB ON A CHIP
卷 10, 期 5, 页码 589-597出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/b919265d
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资金
- National Institutes of Health [R01 ES016665]
- National Science Foundation (NSF) [DMR0213805]
- Vertex Pharmaceuticals Incorporated to Harvard University
- Center for Nanoscale Systems (CNS)
- National Nanotechnology Infrastructure Network (NNIN)
- NSF [ECS-0335765]
- NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES [R01ES016665] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON AGING [R01AG034994] Funding Source: NIH RePORTER
This article describes the fabrication of a microfluidic device for the liquid culture of many individual nematode worms (Caenorhabditis elegans) in separate chambers. Each chamber houses a single worm from the fourth larval stage until death, and enables examination of a population of individual worms for their entire adult lifespans. Adjacent to the chambers, the device includes microfluidic worm clamps, which enable periodic, temporary immobilization of each worm. The device made it possible to track changes in body size and locomotion in individual worms throughout their lifespans. This ability to perform longitudinal measurements within the device enabled the identification of age-related phenotypic changes that correlate with lifespan in C. elegans.
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