期刊
LAB ON A CHIP
卷 9, 期 10, 页码 1371-1376出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/b819080a
关键词
-
类别
资金
- Natural Sciences and Engineering Research Council of Canada (NSERC)
We present a hybrid optical filter design that combines interference and absorbing components For enhanced fluorescence detection in miniaturized highly-integrated lab-on-a-chip devices. The filter is designed in such a way that the advantages of each technology are used to offset the disadvantages of the other. The filter is fabricated with microfabrication compatible processes and materials for monolithic integration with microelectronics and microfluidics devices. The particular embodiment of the filter described herein is designed to discriminate fluorescence emission at 650 nm from excitation at 532 rim. The 9-layer interference filter component is fabricated with alternating TiO2 and SiO2 thin-film layers and has all attenuation of -12.6 dB at 532 nm, and -0.76 dB at 650 inn. The absorbing filter component is fabricated using a dyed photopolymer (KMPR + Orasol Red) having all attenuation of -32.6 dB at 532 nm and -1.28 dB at 650 nm. The total rejection ratio of the hybrid filter is 43 dB. The filter exhibits very low autofluorescence and performs equally well at off-axis incidence angles.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据