期刊
LAB ON A CHIP
卷 9, 期 1, 页码 151-157出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/b807345g
关键词
-
类别
资金
- National Institute of Neurological Disorders and Stroke [1R21NS058646-01]
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R21NS058646] Funding Source: NIH RePORTER
We present two microfluidic approaches for immobilizing the roundworm C. elegans on-chip. The first approach creates a CO2 micro-environment while the second one utilizes a deformable PDMS membrane to mechanically restrict the worm's movement. An on-chip 'behavior' module was used to characterize the effect of these methods on the worm's locomotion pattern. Our results indicate that both methods are appropriate for the short-term (minutes) worm immobilization. The CO2 method offers the additional advantages of long-term immobilization (1-2 hours) and reduced photobleaching, if fluorescent imaging during immobilization is required. We envision the use of these methods in a wide variety of biological studies in C. elegans, including cell developmental and neuronal regeneration studies.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据