期刊
LAB ON A CHIP
卷 9, 期 10, 页码 1412-1421出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/b819362b
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资金
- Programme Interdisciplinaire de Recherche (PIR)
- Agence Nationale pour la Recherche (ANR)
- European Union (EU) Network of Excellence [FP6-500274-1]
- CNRS
- Region Alsace
- EU thematic network 'NetMED: Virtual Institute on Micromechatronics for biomedical industry' [G7RT-CT-2002-05113]
- Marie Curie European Reintegration [MERG-CT-2004-004898]
Microfluidic devices were designed to perform on micromoles of biological macromolecules and viruses the search and the optimization of crystallization conditions by counter-diffusion, as well as the on-chip analysis of crystals by X-ray diffraction. Chips composed of microchannels were fabricated in poly-dimethylsiloxane (PDMS), poly-methyl-methacrylate (PMMA) and cyclo-olefin-copolymer (COC) by three distinct methods, namely replica casting, laser ablation and hot embossing. The geometry of the channels was chosen to ensure that crystallization occurs in a convection-free environment. The transparency of the materials is compatible with crystal growth monitoring by optical microscopy. The quality of the protein 3D structures derived from on-chip crystal analysis by X-ray diffraction using a synchrotron radiation was used to identify the most appropriate polymers. Altogether the results demonstrate that for a novel biomolecule. all steps from the initial search of crystallization conditions to X-ray diffraction data collection for 3D structure determination can be performed in a single chip.
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