期刊
LAB ON A CHIP
卷 8, 期 7, 页码 1056-1061出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/b804234a
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资金
- NHLBI NIH HHS [R01 HL092836-01A1, R01 HL092836] Funding Source: Medline
- NIBIB NIH HHS [R21 EB007249, R21 EB007249-02] Funding Source: Medline
- NIDCR NIH HHS [RL1 DE019024, RL1 DE019024-02, RL1 DE019024-03] Funding Source: Medline
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL092836] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [R21EB007249] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF DENTAL &CRANIOFACIAL RESEARCH [RL1DE019024] Funding Source: NIH RePORTER
Encapsulating cells within hydrogels is important for generating three-dimensional (3D) tissue constructs for drug delivery and tissue engineering. This paper describes, for the first time, the fabrication of large numbers of cell-laden microgel particles using a continuous microfluidic process called stop-flow lithography (SFL). Prepolymer solution containing cells was flowed through a microfluidic device and arrays of individual particles were repeatedly defined using pulses of UV light through a transparency mask. Unlike photolithography, SFL can be used to synthesize microgel particles continuously while maintaining control over particle size, shape and anisotropy. Therefore, SFL may become a useful tool for generating cell-laden microgels for various biomedical applications.
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