期刊
APPLIED SOIL ECOLOGY
卷 85, 期 -, 页码 50-55出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.apsoil.2014.09.002
关键词
Bioremediation; Cr(VI)-contaminated soil; Community dynamic; RFLP; Real-time quantitative PCR
类别
资金
- National Natural Science Foundation for Young Scientists of China [51304250]
- Major science and technology project in Hunan Province [2012FJ1010]
Pannonibacter phragmitetus BB was utilized to intensify the remediation of Cr(VI)-contaminated soil. The dynamics of indigenous bacterial community and the role of exogenous P. phragmitetus BB in the bioremediation process were investigated. Water soluble Cr(VI) and pH of the contaminated soil were 518.84 and 8.64 mg kg (1), respectively. Cr(VI) in contaminated soil and leachate were completely reduced within two days. Restriction fragment length polymorphism (RFLP) revealed that the relative abundance of P. phragmitetus BB ranged from 35.5 to 74.8% during the bioremediation process, which was dominant in the soil bacterial community. Real-time quantitative PCR revealed that the population of P. phragmitetus BB in soil maintained relatively stable within the first 48 h (about 2.0 x 10(9) cells per gram soil), and the concentration of P. phragmitetus BB increased dramatically after the first 48 h. The results suggest that P. phragmitetus BB can play a key role and get competitive advantages in the remediation Cr (VI) contaminated soil. (C) 2014 Elsevier B.V. All rights reserved.
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