期刊
KOREAN JOURNAL OF CHEMICAL ENGINEERING
卷 30, 期 6, 页码 1335-1338出版社
KOREAN INSTITUTE CHEMICAL ENGINEERS
DOI: 10.1007/s11814-013-0058-z
关键词
Biodiesel; Enzymatic Process; Co-immobilization; Lipase
资金
- Advanced Biomass R&D Center (ABC) of Korea
- Ministry of Education, Science, and Technology [2010-0029799]
- National Research Foundation of Korea [2011-0031360] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Candida rugosa lipase and Ryzopus oryzae lipase were simultaneously immobilized on silica gel following enzyme pretreatment. The factors affecting the co-immobilization process, such as reaction time and enzyme ratio, were investigated. Biodiesel was then produced by using the co-immobilized enzyme matrix. A batch system was employed with stepwise methanol feeding, and the continuous process involved a packed-bed reactor. Under optimal immobilization conditions, the activity was approximately 16,000 U/g center dot matrix. When co-immobilized enzyme was used with optimized stepwise methanol feeding, conversion of biodiesel reached about 99% at 3 h and was maintained at a level of over 90% for about 30 reuses.
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