期刊
KOREAN JOURNAL OF CHEMICAL ENGINEERING
卷 28, 期 4, 页码 1078-1084出版社
KOREAN INSTITUTE CHEMICAL ENGINEERS
DOI: 10.1007/s11814-010-0483-1
关键词
NADH; Torulopsis glabrata; Pyruvate; H2O-forming NADH Oxidase; Alternative Oxidase
资金
- National Science Foundation for Distinguished Young Scholars [20625619]
- National Natural Science Foundation of China [20706025]
- Key Project of China National Programs for Fundamental Research and Development [2007CB714303]
- Foundation for the Author of National Excellent Doctoral Dissertation of China [200962]
The aim of this study was to increase pyruvate production efficiency in a multi-vitamin auxotrophic yeast, Torulopsis glabrata. This was achieved by decreasing intracellular NADH content through the introduction of two different NADH reoxidation pathways: one in the cytoplasm and the other in mitochondria A nox (encoding a cytoplasmic H2O-forming NADH oxidase) and an AOXI (encoding a mitochondrial alternative oxidase) were successfully expressed heterologously in T. glabrata, resulting in a decrease in the NADH and ATP contents of 55% and 26%, (in T. glabrata NOX) and 45% and 47% (in T. glabrata AOX), respectively. The decreases in nucleotide concentrations led to increases in the glucose consumption rate, the pyruvate yield and pyruvate productivity of 27%, 15% and 22% (in T. glabrata NOX) and 38%, 21%, and 29% (in T. glabrata AOX), respectively, compared with the corresponding values of the control. We conclude that this method provides an alternative way to enhance the production efficiency of NADH-related metabolites.
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