FcγRIIB regulates T-cell autoreactivity, ANCA production, and neutrophil activation to suppress anti-myeloperoxidase glomerulonephritis

Anti-neutrophil cytoplasmic antibody (ANCA)-associated glomerulonephritis involves innate and adaptive immune cells in the induction of autoimmunity and in autoimmune effector responses. Most Fc gamma receptors (Fc gamma Rs) activate immune cells, but Fc gamma RIIB, found in humans and mice on B cells and innate cells, is an inhibitory receptor. Here we tested whether endogenous Fc gamma RIIB negatively regulates autoreactivity and effector responses in experimental anti-myeloperoxidase (MPO) glomerulonephritis, using wild-type and Fc gamma RIIB-/- mice. After MPO immunization, Fc gamma RIIB-/- mice developed higher MPO-ANCA titers and increased anti-MPO T-cell responses. Transfer of Fc gamma RIIB-deficient dendritic cells loaded with a nephritogenic MPO peptide (MPO409-428) into wild-type mice induced stronger autoimmunity than dendritic cells derived from wild-type mice. Transferring anti-MPO antibodies into lipopolysaccharide-primed mice resulted in increased glomerular neutrophil accumulation and injury in Fc gamma RIIB-/- mice, showing a role for Fc gamma RIIB in suppressing neutrophil activation. Inducing active autoimmunity to MPO followed by triggering T cell-mediated glomerular injury by transfer of sub-nephritogenic doses of lipopolysaccharide and anti-MPO antibodies resulted in more disease in Fc gamma RIIB-/- mice. Thus, endogenous Fc gamma RIIB negatively regulates anti-MPO autoimmunity and glomerulonephritis by dendritic cells, B cells, and neutrophils to limit MPO-ANCA production, T-cell responses, and neutrophil activation.