期刊
KIDNEY INTERNATIONAL
卷 82, 期 4, 页码 412-427出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ki.2012.105
关键词
acute kidney injury; exosome; ischemia-reperfusion
资金
- Fresenius Medical Care
- Italian Government Miur PRIN project
- Regione Piemonte, Piattaforme Biotecnologiche PiSTEM project
- Converging Technologies NanoIGT project
- Ricerca Finalizzata and Local University Grants
Endothelial progenitor cells are known to reverse acute kidney injury by paracrine mechanisms. We previously found that microvesicles released from these progenitor cells activate an angiogenic program in endothelial cells by horizontal mRNA transfer. Here, we tested whether these microvesicles prevent acute kidney injury in a rat model of ischemia-reperfusion injury. The RNA content of microvesicles was enriched in microRNAs (miRNAs) that modulate proliferation, angiogenesis, and apoptosis. After intravenous injection following ischemia-reperfusion, the microvesicles were localized within peritubular capillaries and tubular cells. This conferred functional and morphologic protection from acute kidney injury by enhanced tubular cell proliferation, reduced apoptosis, and leukocyte infiltration. Microvesicles also protected against progression of chronic kidney damage by inhibiting capillary rarefaction, glomerulosclerosis, and tubulointerstitial fibrosis. The renoprotective effect of microvesicles was lost after treatment with RNase, nonspecific miRNA depletion of microvesicles by Dicer knock-down in the progenitor cells, or depletion of pro-angiogenic miR-126 and miR-296 by transfection with specific miR-antagomirs. Thus, microvesicles derived from endothelial progenitor cells protect the kidney from ischemic acute injury by delivering their RNA content, the miRNA cargo of which contributes to reprogramming hypoxic resident renal cells to a regenerative program. Kidney International (2012) 82, 412-427; doi:10.1038/ki.2012.105; published online 11 April 2012
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