期刊
KIDNEY INTERNATIONAL
卷 81, 期 8, 页码 751-761出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ki.2011.450
关键词
cell death; ischemia; ischemia-reperfusion; ischemic renal failure; tubule cells
资金
- Dr Werner Jackstadt-Stiftung
- German Society of Nephrology
- European Nephrology and Dialysis Institute
- Fresenius Medical Care Deutschland GmbH (SK)
- Else Kroner-Fresenius Stiftung (UK)
- Fresenius Medical Care Deutschland GmbH (UK)
Loss of kidney function in renal ischemia/reperfusion injury is due to programmed cell death, but the contribution of necroptosis, a newly discovered form of programmed necrosis, has not been evaluated. Here, we identified the presence of death receptor-mediated but caspase-independent cell death in murine tubular cells and characterized it as necroptosis by the addition of necrostatin-1, a highly specific receptor-interacting protein kinase 1 inhibitor. The detection of receptor-interacting protein kinase 1 and 3 in whole-kidney lysates and freshly isolated murine proximal tubules led us to investigate the contribution of necroptosis in a mouse model of renal ischemia/reperfusion injury. Treatment with necrostatin-1 reduced organ damage and renal failure, even when administered after reperfusion, resulting in a significant survival benefit in a model of lethal renal ischemia/reperfusion injury. Unexpectedly, specific blockade of apoptosis by zVAD, a pan-caspase inhibitor, did not prevent the organ damage or the increase in urea and creatinine in vivo in renal ischemia/reperfusion injury. Thus, necroptosis is present and has functional relevance in the pathophysiological course of ischemic kidney injury and shows the predominance of necroptosis over apoptosis in this setting. Necrostatin-1 may have therapeutic potential to prevent and treat renal ischemia/reperfusion injury. Kidney International (2012) 81, 751-761; doi:10.1038/ki.2011.450; published online 11 January 2012
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