期刊
KIDNEY INTERNATIONAL
卷 74, 期 6, 页码 808-816出版社
ELSEVIER SCIENCE INC
DOI: 10.1038/ki.2008.330
关键词
integrin; migration; peritoneal mesothelial cells; T cells; alpha 4 beta 1; alpha 6 beta 1
资金
- National Science Council [94-2314-B-075-044, 95-2314-B-075-069-MY2]
We previously showed that a local immune response largely composed of type 1 T cells correlated with a favorable outcome of the peritonitis associated with peritoneal dialysis. To clarify how these subsets are recruited to the peritoneal cavity during inflammation, we measured integrin-mediated interactions between the T cells and human peritoneal mesothelial cells. Direct microscopy showed that lipopolysaccharide or peritoneal dialysis effluent stimulated the adherence of T cells to mesothelial cells, a process mediated by the integrins alpha 6 beta 1 and alpha 4 beta 1. Further, the migration of Th1 cell across human mesothelial cell monolayers grown on transwell surfaces was reduced by anti-alpha 6 beta 1 integrin antibody while that of Th2 cell was inhibited by an anti-alpha 4 integrin antibody. Pretreatment with either lipopolysaccharide or rapid response peritoneal dialysis effluent stimulated T cell migration and this was significantly decreased by the alpha 6 beta 1 compared to the alpha 4 antibody. These results suggest that integrins may play an important role in mediating selective T cell subset adhesion and migration across human peritoneal mesothelial cell monolayers and differential integrin expression and selective T cell subset recruitment during peritonitis may affect outcome.
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