期刊
JPC-JOURNAL OF PLANAR CHROMATOGRAPHY-MODERN TLC
卷 24, 期 6, 页码 470-474出版社
SPRINGER HEIDELBERG
DOI: 10.1556/JPC.24.2011.6.3
关键词
Genus Peganum; TLC-bioautographic assay; Acetylcholinesterase inhibitors; Harmine; Harmaline
资金
- National Natural Science Foundation of China [U1130303]
- Xinjiang Uygur Autonomous Region of China [U1130303]
- National Nature Science Foundation of China [81173119]
- Ministry of Science and Technology of China [2012ZX09103201-051]
- Shanghai Science & Technology Development Foundation [08JC1418600, 08DZ1972101]
In traditional Chinese medicine, plants of the genus Peganum have been used to treat cough, hypertension, diabetes, asthma, jaundice, lumbago, and many other ailments. In this study, seeds of the plants of genus Peganum, including P. harmala Linn., P. multisectum (Maxim) Bohr, P. nigellastrum Bunge, and Peganum variety were collected from different provinces in China. A simple, rapid, and effective thin-layer chromatographic (TLC) fingerprint combined with bioautographic technique has been established for the identification of acetylcholinesterase (AChE) inhibitors from these seeds. The methanol extracts of seeds were separated on silica gel plates with ethyl acetate-methanol-ammonia 10:1.5:0.5 (v/v) as mobile phase, and then the plates were inspected under UV 366 nm and visualized by spraying with both Dragendorff's and vanillin-sulfuric acid reagents as well as by bioautographic assay. Moreover, the limits on AChE inhibitive activity of harmine and harmaline were found to be 0.01 mu g, in comparison to that of galanthamine of also 0.01 mu g. The TLC fingerprints combined with the bioautographic method could distinguish the seeds of the different species of genus Peganum investigated. Moreover, harmine and harmaline displayed similar AChE inhibition compared to galanthamine.
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