期刊
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
卷 65, 期 8, 页码 832-842出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/gerona/glq081
关键词
Mitochondrial aging; Exercise; Respiratory chain functionality; BN-PAGE; Two-dimensional electrophoresis
资金
- Fundacao para a Ciencia e Tecnologia [PTDC/DES/70757/2006, REDE/1505/REM/2005]
- [SFRH/BPD/24158/2005]
- Fundação para a Ciência e a Tecnologia [PTDC/DES/70757/2006, SFRH/BPD/24158/2005] Funding Source: FCT
The aim of this study was to investigate the influence of lifestyle on the aging alterations in skeletal muscle mitochondrial proteins. Thirty C57BL/6 strain mice (2 months) were randomly divided into three groups (young, Y; old sedentary, S; and old active, A). The S and A mice were individually placed into standard cages and in cages with running wheels for 25 months. Upon killing, mitochondria from the hind limb skeletal muscles were isolated for the evaluation of general proteome alterations, carbonylation, and electron transport chain (ETC) activity. We identified 77 different proteins mostly from the oxidative phosphorylation and mitochondrial metabolism. Sedentary mice presented a significant loss of ETC functionality in opposition to active mice. Although some proteins were found damaged in both A and S mice, damage to ETC proteins was more evident in S. Moreover, it is also possible to conclude that lifestyle is a key modulator for preventing the aging-induced protein expression and functionality in mitochondria.
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