期刊
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
卷 13, 期 6, 页码 487-493出版社
ZHEJIANG UNIV
DOI: 10.1631/jzus.B1100298
关键词
Caffeic acid; Purification; Post-processing; Copper tolerance; Elsholtzia splendens
类别
资金
- Zhejiang Provincial Qianjiang Talents for Science and Technology [2011R10026]
- Education Department of Zhejiang Province [Y201016563]
- Research Funds from State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering [2009490711]
- Fundamental Research Funds for the Central Universities, China
In the current study, caffeic acid was an important metabolite in the highly copper-tolerant plant Elsholtzia splendens. Preparation and purification of caffeic acid were performed on the dried biomass of the plants by means of sonication/ethanol extraction, followed by purification using a macroporous resin (D101 type) column and silica gel chromatography. The faint-yellow caffeic acid product was yielded with a purity of 98.46%, and it was chemically identified from spectra of Fourier transform infrared spectroscopy (FTIR), proton nuclear magnetic resonance (H-1 NMR)/carbon nuclear magnetic resonance (C-13 NMR), and electrospray ionization mass spectrometry (ESI-MS). Caffeic acid is a possible product from the post-harvest processing of Elsholtzia splendens biomass.
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