期刊
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
卷 13, 期 1, 页码 20-28出版社
ZHEJIANG UNIV
DOI: 10.1631/jzus.B1100117
关键词
Mesenchymal stem cells (MSCs); Proliferation; Differentiation; Insulin-like growth factor-1 (IGF-1); CXC chemokine receptor 4 (CXCR4); Migration
类别
资金
- Guangdong Provincial Natural Science Foundation of China [8151008901000157]
- Scientific Research Fund of Guangdong Province, China [2008B030301045, 2011B031800021]
- Health Ministry of Guangdong Province, China [B2011310]
Objective: To explore the effects of insulin-like growth factor-1 (IGF-1) on migration, proliferation and differentiation of mesenchymal stem cells (MSCs). Methods: MSCs were obtained from Sprague-Dawley rats by a combination of gradient centrifugation and cell culture techniques and treated with IGF-1 at concentrations of 5-20 ng/ml. Proliferation of MSCs was determined as the mean doubling time. Expression of CXC chemokine receptor 4 (CXCR4) and migration property were determined by flow cytometry and transwell migration essay, respectively. mRNA expression of GATA-4 and collagen II was determined by reverse transcription-polymerase chain reaction (RT-PCR). Results: The mean doubling time of MSC proliferation was decreased, and the expression of CXCR4 on MSCs and migration of MSCs were increased by IGF-1, all in a dose-dependent manner, while the optimal concentration of IGE-1 on proliferation and migration was different. IGF-1 did not affect the expression of GATA-4 or collagen II mRNA. Conclusions: IGF-1 dose-dependently stimulated the proliferation of MSCs, upregulated the expression of CXCR4, and accelerated migration. There was no apparent differentiation of MSCs to cardiomyocytes or chondrocytes after culturing with IGF-1 alone.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据