期刊
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
卷 11, 期 6, 页码 391-401出版社
ZHEJIANG UNIV
DOI: 10.1631/jzus.B1000056
关键词
Leptotrichia hongkongensis; Bacterium; Blood culture; Anaerobic bacteria; Novel species
类别
资金
- Hong Kong Special Administrative Region of China
- Research Grant Council
- University Development Fund
- Outstanding Young Researcher Award
- Committee for Research and Conference Grant
- University of Hong Kong, China
A straight, non-sporulating, Gram-variable bacillus (HKU24(T)) was recovered from the blood culture of a patient with metastatic breast carcinoma. After repeated subculturing in BACTEC Plus Anaerobic/F blood culture broth, HKU24(T) grew on brucella agar as non-hemolytic, pinpoint colonies after 96 h of incubation at 37 A degrees C in an anaerobic environment and aerobic environment with 5% CO2. Growth was enhanced with a streak of Staphylococcus aureus. HKU24(T) was non-motile and catalase-negative, but positive for alkaline phosphatase, beta-glucosidase, and alpha-glucosidase. It hydrolyzed phenylphosphonate and reduced resazurin. 16S rRNA, groEL, gyrB, recA, and rpoB sequencing showed that HKU24(T) occupies a distinct phylogenetic position among the Leptotrichia species, being most closely related to Leptotrichia trevisanii. Using HKU24(T) groEL, gyrB, recA, and rpoB gene-specific primers, fragments of these genes were amplified from one of 20 oral specimens. Based on phenotypic and genotypic characteristics, we propose a new species, Leptotrichia hongkongensis sp. nov., to describe this bacterium.
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