期刊
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
卷 10, 期 2, 页码 93-102出版社
ZHEJIANG UNIV
DOI: 10.1631/jzus.B0820238
关键词
Curcumin; Apoptosis; Mitochondrial pathway; HT-29 cells
类别
资金
- Science and Technology Department of Zhejiang Province, China [2006C12098]
To investigate the effects of curcumin on release of cytochrome c and expressions of Bcl-2, Bax, Bad, Bcl-xL, caspase-3, poly ADP-ribose polymerase (PARP), and survivin of HT-29 cells. Methods: HT-29 cells were treated with curcumin (0 similar to 80 mu mol/L) for 24 h. The release of cytochrome c from the mitochondria and the apoptosis-related proteins Bax, Bcl-2, Bcl-xL, Bad, caspase-3, PARP, and survivin were determined by Western blot analysis and their mRNA expressions by reverse transcriptase-polymerase chain reaction (RT-PCR). Curcumin significantly induced the growth inhibition and apoptosis of HT-29 cells. A decrease in expressions of Bcl-2, Bcl-xL and survivin was observed after exposure to 10 similar to 80 mu mol/L curcumin, while the levels of Bax and Bad increased in the curcumin-treated cells. Curcumin also induced the release of cytochrome c, the activation of caspase-3, and the cleavage of PARP in a dose-dependent manner. These data suggest that curcumin induced the HT-29 cell apoptosis possibly via the mitochondria-mediated pathway.
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