期刊
PROTEOMICS CLINICAL APPLICATIONS
卷 9, 期 3-4, 页码 277-288出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/prca.201400119
关键词
2DE; DIGE; Gel electrophoresis; MS
资金
- Ovarian Cancer Research Foundation (OCRF)
- Bioplatforms Australia
- Government of South Australia
Difference gel electrophoresis enables the accurate quantification of changes in the proteome including combinations of PTMs and protein isoform expression. Here, we review recent advances in study design, image acquisition, and statistical analysis. We also compare DIGE to established and emerging mass spectrometric analysis technologies. Despite these recent advances in MS and the still unsolved limitations of 2DE to map hydrophobic, high molecular weight proteins with extreme pIs, DIGE remains the most comprehensive top-down method to study changes in abundance of intact proteins.
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