期刊
PROTEOMICS
卷 15, 期 4, 页码 773-786出版社
WILEY-BLACKWELL
DOI: 10.1002/pmic.201300494
关键词
2DE; Cell biology; Inflammation; Macrophage polarization; Proteomic analysis; THP-1 cell
资金
- National Basic Research Program of China (973 Program) [2011CB935803]
- National Natural Science Foundation of China [81071712, 81272311]
- Specialized Research Fund for the Doctoral Program of Higher Education [20130171110056]
Macrophages are heterogeneous and plastic populations that are an essential component of inflammation and host defense. To understand how macrophages respond to cytokine signals, we used 2DE to identify protein profiles in macrophages stimulated with interleukin 4 (M2) and those stimulated with lipopolysaccharide and interferon gamma (M1). In total, 32 differentially expressed proteins in THP-1 cells were identified by MALDI-TOF MS/MS analysis. The different proteins were mainly involved in cellular structure, protein metabolism, stress response, oxidative response, and nitric oxide production during macrophage polarization. In particular, proteins playing important roles in production of nitric oxide (NO) were downregulated in M2 macrophages. Many antioxidant and heat shock proteins, which are related to oxidative response, were upregulated in M2 macrophages. More importantly, a remarkable decrease in intracellular ROS and NO production were detected in M2 macrophages. Our results provide a proteomic profile of differentially polarized macrophages and validate the function of the identified proteins, which may indicate possible mechanism of macrophage polarization process.
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