期刊
JOURNAL OF WOOD SCIENCE
卷 54, 期 4, 页码 312-317出版社
SPRINGER TOKYO
DOI: 10.1007/s10086-008-0958-4
关键词
At5g54160; 5-hydroxyconiferaldehyde O-methyltransferase; lignin
The function of an Arabidopsis thaliana gene, At5g54160 annotated as a caffeic acid O-methyltransferase CAOMT gene was characterized. The recombinant enzyme of this gene (AtOMT1) catalyzed the O-methylation of phenylpropanoid and flavonoid substrates. The specificity constants (k (cat)/K (m)) for 5-hydroxyconiferaldehyde (5-HCAld) and quercetin were both 0.11 mu M(-1).min(-1). On the other hand, lignins of At5g54160-knockout Arabidopsis mutants lacked syringyl units. In addition, we showed that the gene silencing also resulted in significant accumulation of caffeyl alcohol (CaAlc). These results strongly suggested that At5g54160 gene is involved in syringyl lignin synthesis for the methylation of both 5-hydroxyconiferaldehyde and 3,4-dihydroxyphenyl compound(s).
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