期刊
PROTEOMICS
卷 15, 期 23-24, 页码 3969-3979出版社
WILEY-BLACKWELL
DOI: 10.1002/pmic.201500256
关键词
Crustacean; Isotopic labeling; Mass spectrometry imaging; Neuropeptides; Salinity stress
资金
- National Science Foundation [CHE-1413596]
- National Institutes of Health [R01DK071801]
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [1413596] Funding Source: National Science Foundation
Environmental fluctuations, such as salinity, impose serious challenges to marine animal survival. Neuropeptides, signaling molecules involved in the regulation process, and the dynamic changes of their full complement in the stress response have yet to be investigated. Here, a MALDI-MS-based stable isotope labeling quantitation strategy was used to investigate the relationship between neuropeptide expression and adaptability of Carcinus maenas to various salinity levels, including high (60 parts per thousand [p.p.t.]) and low (0 p.p.t.) salinity, in both the crustacean pericardial organ (PO) and brain. Moreover, a high salinity stress time course study was conducted. MS imaging (MSI) of neuropeptide localization in C. maenas PO was also performed. As a result of salinity stress, multiple neuropeptide families exhibited changes in their relative abundances, including RF amides (e.g. APQGNFLRFamide), RYamides (e.g. SSFRVGGSRYamide), B-type allatostatins (AST-B; e.g. VPNDWAHFRGSWamide), and orcokinins (e.g. NFDEIDRSSFGFV). The MSI data revealed distribution differences in several neuropeptides (e.g. SGFYANRYamide) between color morphs, but salinity stress appeared to not have a major effect on the localization of the neuropeptides.
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