期刊
PROTEIN EXPRESSION AND PURIFICATION
卷 110, 期 -, 页码 7-13出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pep.2014.12.015
关键词
Plant; Pyruvate kinase; Respiratory metabolism; Glycolysis; Recombinant protein; Chaperone
类别
资金
- Discovery Grant from the National Science and Engineering Research Council of Canada (NSERC) [RGPIN 227271]
- NSERC Undergraduate Student Research Award
- Fonds Quebecois de Recherche Nature et Technologie from the government of Quebec
The cDNA encoding for a Solanum tuberosum cytosolic pyruvate kinase 1 (PKc1) highly expressed in tuber tissue was cloned in the bacterial expression vector pProEX HTc. The construct carried a hexahistidine tag in N-terminal position to facilitate purification of the recombinant protein. Production of high levels of soluble recombinant PKc1 in Escherichia coli was only possible when using a co-expression strategy with the chaperones GroES-GroEL. Purification of the protein by Ni2+ chelation chromatography yielded a single protein with an apparent molecular mass of 58 kDa and a specific activity of 34 units mg(-1) protein. The recombinant enzyme had an optimum pH between 6 and 7. It was relatively heat stable as it retained 80% of its activity after 2 min at 75 degrees C. Hyperbolic saturation kinetics were observed with ADP and UDP whereas sigmoidal saturation was observed during analysis of phosphoenolpyruvate binding. Among possible effectors tested, aspartate and glutamate had no effect on enzyme activity, whereas alpha-ketoglutarate and citrate were the most potent inhibitors. When tested on phosphoenolpyruvate saturation kinetics, these latter compounds increased S-0.5. These findings suggest that S. tuberosum PKc1 is subject to a strong control by respiratory metabolism exerted via citrate and other tricarboxylic acid cycle intermediates. (C) 2014 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据