期刊
JOURNAL OF VIROLOGY
卷 87, 期 4, 页码 2072-2080出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.02807-12
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资金
- National Natural Science Foundation of China [31201921]
N-pro is a multifunctional autoprotease unique to pestiviruses. The interacting partners of the N-pro protein of classical swine fever virus (CSFV), a swine pestivirus, have been insufficiently defined. Using a yeast two-hybrid screen, we identified poly(C)-binding protein 1 (PCBP1) as a novel interacting partner of the CSFV N-pro protein and confirmed this by coimmunoprecipitation, glutathione S-transferase (GST) pulldown, and confocal assays. Knockdown of PCBP1 by small interfering RNA suppressed CSFV growth, while overexpression of PCBP1 promoted CSFV growth. Furthermore, we showed that type I interferon was downregulated by PCBP1, as well as N-pro. Our results suggest that cellular PCBP1 positively modulates CSFV growth.
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