Complete Genome Sequence of T4-Like Escherichia coli Bacteriophage HX01

Phage T4 is among the best-characterized biological systems (S. Kanamaru and F. Arisaka, Seikagaku 74: 131-135, 2002; E. S. Miller et al., Microbiol. Mol. Biol. Rev. 67: 86-156, 2003; W. B. Wood and H. R. Revel, Bacteriol. Rev. 40: 847-868, 1976). To date, several genomes of T4-like bacteriophages are available in public databases but without any APEC bacteriophages (H. Jiang et al., Arch. Virol. 156: 1489-1492, 2011; L. Kaliniene, V. Klausa, A. Zajanckauskaite, R. Nivinskas, and L. Truncaite, Arch. Virol. 156: 1913-1916, 2011; J. H. Kim et al., Vet. Microbiol. 157: 164-171, 2012; W. C. Liao et al., J. Virol. 85: 6567-6578, 2011). We isolated a bacteriophage from a duck factory, named HX01, that infects avian pathogenic Escherichia coli (APEC). Sequence and morphological analyses revealed that phage HX01 is a T4-like bacteriophage and belongs to the family Myoviridae. Here, we announce the complete genome sequence of phage HX01 and report the results of our analysis.