期刊
JOURNAL OF VIROLOGY
卷 86, 期 8, 页码 4123-4128出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.06232-11
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资金
- Japan Initiative for Global Research Network on Infectious Diseases from the Ministries of Education, Culture, Sports, Science, and Technology
- Health, Labor, and Welfare of Japan, by ERATO (Japan Science and Technology Agency)
- National Institute of Allergy and Infectious Diseases Public Health Service
- Otsuka Toshimi International Scholarship Foundation
- Honjo International Scholarship Foundations
- Grants-in-Aid for Scientific Research [22790425] Funding Source: KAKEN
Vaccination is the primary form of protection from influenza virus infection. We recently developed a replication-incompetent PB2-knockout (PB2-KO) influenza virus that possesses a reporter gene (the green fluorescent protein gene) in the coding region of the PB2 segment. This virus replicated to high titers in PB2-expressing, but not unmodified, cells, suggesting its potential safety and feasibility as a vaccine. Here, we tested its efficacy in a murine model. The levels of IgG and IgA antibodies against influenza virus in sera, nasal washes, and bronchoalveolar lavage fluids of mice immunized with the PB2-KO virus were higher than those induced by a conventional inactivated vaccine. All PB2-KO virus-immunized mice survived challenges with lethal doses of influenza virus. Moreover, importantly, mice immunized with the PB2-KO virus produced antibodies against the reporter protein, suggesting that the PB2-KO virus has potential as a multivalent vaccine to combat infection with not only influenza virus but also other pathogens.
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