期刊
JOURNAL OF VIROLOGY
卷 86, 期 2, 页码 1250-1254出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.06367-11
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资金
- Burroughs Wellcome Fund Clinical Scientist Award in Translational Research
- NRSA [F32AI082930]
- Soros, MedScholars and HHMI Fellowship
- [RO1AI087917]
MicroRNA-122 (miR-122) enhances hepatitis C virus (HCV) fitness via targeting two sites in the 5'-untranslated region (UTR) of HCV. We used selective 2'-hydroxyl acylation analyzed by primer extension to resolve the HCV 5'-UTR's RNA secondary structure in the presence of miR-122. Nearly all nucleotides in miR-122 are involved in targeting the second site, beyond classic seed base pairings. These additional interactions enhance HCV replication in cell culture. To our knowledge, this is the first biophysical study of this complex to reveal the importance of 'tail' miR-122 nucleotide interactions.
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