期刊
JOURNAL OF VIROLOGY
卷 84, 期 19, 页码 10289-10296出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.01304-10
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资金
- U.S. Embassy in Cameroon
- NIH [DP1-OD000370]
- google.org
- Skoll Foundation
- Henry M. Jackson Foundation for the Advancement of Military Medicine
- Global Emerging Infections Surveillance and Response System (GEIS)-a Division of the United States Armed Forces Health Surveillance Center
- United States Agency for International Development (USAID) [GHN-A-OO-09-00010-00]
- National Institutes of Health [R01 AI50529]
- Agence National de Recherches sur le SIDA, France [ANRS 12125, ANRS 12182]
- Institut de Recherche pour le Developpement (IRD)
- Helsinki University Central Hospital
- Sigrid Juselius Foundation
- Medical Society of Finland (FLS)
- Academy of Finland [1122539]
- Baxter (Los Angeles, CA)
- Royal Society
- Medical Research Council [G0900740] Funding Source: researchfish
- MRC [G0900740] Funding Source: UKRI
Infections with human parvoviruses B19 and recently discovered human bocaviruses (HBoVs) are widespread, while PARV4 infections are transmitted parenterally and prevalent specifically in injecting drug users and hemophiliacs. To investigate the exposure and circulation of parvoviruses related to B19 virus, PARV4, and HBoV in nonhuman primates, plasma samples collected from 73 Cameroonian wild-caught chimpanzees and gorillas and 91 Old World monkey (OWM) species were screened for antibodies to recombinant B19 virus, PARV4, and HBoV VP2 antigens by enzyme-linked immunosorbent assay (ELISA). Moderate to high frequencies of seroreactivity to PARV4 (63% and 18% in chimpanzees and gorillas, respectively), HBoV (73% and 36%), and B19 virus (8% and 27%) were recorded for apes, while OWMs were uniformly negative (for PARV4 and B19 virus) or infrequently reactive (3% for HBoV). For genetic characterization, plasma samples and 54 fecal samples from chimpanzees and gorillas collected from Cameroonian forest floors were screened by PCR with primers conserved within Erythrovirus, Bocavirus, and PARV4 genera. Two plasma samples (chimpanzee and baboon) were positive for PARV4, while four fecal samples were positive for HBoV-like viruses. The chimpanzee PARV4 variant showed 18% and 15% nucleotide sequence divergence in NS and VP1/2, respectively, from human variants (9% and 7% amino acid, respectively), while the baboon variant was substantially more divergent, mirroring host phylogeny. Ape HBoV variants showed complex sequence relationships with human viruses, comprising separate divergent homologues of HBoV1 and the recombinant HBoV3 species in chimpanzees and a novel recombinant species in gorillas. This study provides the first evidence for widespread circulation of parvoviruses in primates and enables future investigations of their epidemiology, host specificity, and (co)evolutionary histories.
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