期刊
JOURNAL OF VIROLOGY
卷 84, 期 22, 页码 11841-11848出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.01153-10
关键词
-
类别
资金
- Cambridge Infectious Diseases Consortium
- DEFRA Veterinary Training and Research Initiative
- DEFRA [SEO420, SEO423, SEO424]
- NIH part of the NIH road map for medical research [DP1-OD000490-01, 223498]
- European Union
- Human Frontier Science Program [P0050/2008]
- Alborada Trust
- Science and Technology Directorate, Department of Homeland Security
- Royal Society, London
- Clare College, Cambridge
All lyssaviruses cause fatal encephalitis in mammals. There is sufficient antigenic variation within the genus to cause variable vaccine efficacy, but this variation is difficult to characterize quantitatively: sequence analysis cannot yet provide detailed antigenic information, and antigenic neutralization data have been refractory to high-resolution robust interpretation. Here, we address these issues by using state-of-the-art antigenic analyses to generate a high-resolution antigenic map of a global panel of 25 lyssaviruses. We compared the calculated antigenic distances with viral glycoprotein ectodomain sequence data. Although 67% of antigenic variation was predictable from the glycoprotein amino acid sequence, there are in some cases substantial differences between genetic and antigenic distances, thus highlighting the risk of inferring antigenic relationships solely from sequence data at this time. These differences included epidemiologically important antigenic differences between vaccine strains and wild-type rabies viruses. Further, we quantitatively assessed the antigenic relationships measured by using rabbit, mouse, and human sera, validating the use of nonhuman experimental animals as a model for determining antigenic variation in humans. The use of passive immune globulin is a crucial component of rabies postexposure prophylaxis, and here we also show that it is possible to predict the reactivity of immune globulin against divergent lyssaviruses.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据