期刊
JOURNAL OF VIROLOGY
卷 83, 期 16, 页码 8289-8292出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.00709-09
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资金
- HIV Vaccine Clinical Trials Network [AI46705]
- Duke Center for AIDS Research [AI64518]
- Bill and Melinda Gates Foundation [38619]
- NIH [CA067358]
The TZM-bl cell line that is commonly used to assess neutralizing antibodies against human immunodeficiency virus type 1 (HIV-1) was recently reported to be contaminated with an ecotropic murine leukemia virus (MLV) (Y. Takeuchi, M. O. McClure, and M. Pizzato, J. Virol. 82: 12585-12588, 2008), raising questions about the validity of results obtained with this cell line. Here we confirm this observation and show that HIV-1 neutralization assays performed with a variety of serologic reagents in a similar cell line that does not harbor MLV yield results that are equivalent to those obtained in TZM-bl cells. We conclude that MLV contamination has no measurable effect on HIV-1 neutralization when TZM-bl cells are used as targets for infection.
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