期刊
JOURNAL OF VIROLOGY
卷 82, 期 6, 页码 3131-3134出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.02266-07
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- NIAID NIH HHS [AI22470, R01 AI048682, T32 AI055432, R01 AI022470, T32 AI007046, T32 AI07076-27, AI48682] Funding Source: Medline
We previously showed that the cysteines flanking the internal fusion peptide of the avian sarcoma/leukosis virus subtype A (ASLV-A) Env (EnvA) are important for infectivity and cell-cell fusion. Here we define the stage of fusion at which the cysteines are required. The flanking cysteines are dispensable for receptor-triggered membrane association but are required for the lipid mixing step of fusion, which, interestingly, displays a high pH onset and a biphasic profile. Second-site mutations that partially restore infection partially restore lipid mixing. These findings indicate that the cysteines flanking the internal fusion peptide of EnvA (and perhaps by analogy Ebola virus glycoprotein) are important for the foldback stage of the conformational changes that lead to membrane merger.
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