期刊
JOURNAL OF VIROLOGICAL METHODS
卷 203, 期 -, 页码 54-64出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2014.03.015
关键词
In vitro replication and protein expression; Huh7 cells; Transfection
资金
- National Research Foundation (GUN) [37665, 65530, 2072786]
- Medical Research Council
- Japan Society for the Promotion of Science
- University of the Witwatersrand, Poliomyelitis Research Foundation [06/05]
- European Molecular Biology Organization [ASTF 209.2009]
- Heinrich-Pette Institute, Leibniz Institute for Experimental Virology and Immunology
- Korea Agency for Infrastructure Technology Advancement (KAIA) [65530] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Hepatitis B virus (HBV) is hyperendemic to southern Africa, with genotype A of HBV being the predominant genotype, and subgenotype A1 prevailing. Infection with this subgenotype is associated with rapid disease progression, and high frequency of hepatocellular carcinoma development. The objectives of our study was to construct recombinant 1.28 mer replication competent HBV DNA plasmids of subgenotypes A1, A2 and D3 containing authentic endogenous HBV promoters and to follow their replication in vitro after transfection of Huh7 cells. We found that subgenotype D3 replicated at a lower level, as measured by HBsAg and HBV DNA levels, when compared to cells transfected with genotype A. There was no difference in the intracellular and extracellular HBsAg between cells transfected with subgenotypes A1 or A2. Cells transfected with subgenotype A1 had higher levels of intracellular replicative intermediates and HBcAg, and lower extracellular expression of HBeAg from days 1 to 3, when compared to cells transfected with subgenotype A2. In conclusion, the generation of these replication competent clones is an important step in the functional characterization of subgenotypes of HBV circulating in Africa and their comparison to strains circulating in other geographical regions of the world. (C) 2014 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license
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