期刊
JOURNAL OF VIROLOGICAL METHODS
卷 209, 期 -, 页码 116-120出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2014.09.004
关键词
Influenza A infection; Inoculation; Mice; Lung injury; Intratracheal; Intranasal
资金
- NCI NIH HHS [P30 CA060553] Funding Source: Medline
- NHLBI NIH HHS [T32 HL076139, R01 HL124664] Funding Source: Medline
- NIAID NIH HHS [F32 AI094976] Funding Source: Medline
- NIEHS NIH HHS [R01 ES013995] Funding Source: Medline
- BLRD VA [I01 BX000201] Funding Source: Medline
Infection of mice with human or murine adapted influenza A viruses results in a severe pneumonia. However, the results of studies from different laboratories show surprising variability, even in genetically similar strains. Differences in inoculum size related to the route of viral delivery (intranasal vs. intratracheal) might explain some of this variability. To test this hypothesis, mice were infected intranasally or intratracheally with different doses of influenza A virus (A/WSN/33 [H1N1]). Daily weights, a requirement for euthanasia, viral load in the lungs and brains, inflammatory cytokines, wet-to-dry ratio, total protein and histopathology of the infected mice were examined. With all doses of influenza tested, intranasal delivery resulted in less severe lung injury, as well as smaller and more variable viral loads in the lungs when compared with intratracheal delivery. Virus was not detected in the brain following either method of delivery. It is concluded that compared to intranasal infection, intratracheal infection with influenza A virus is a more reliable method to deliver virus to the lungs. (C) 2014 Elsevier B.V. All rights reserved.
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