Detection of enterovirus in environmental waters: A new optimized method compared to commercial real-time RT-qPCR kits

Despite the progress in water and wastewater treatment technologies, waterborne diseases are still a major concern of public health. In the reported water-related outbreaks, viruses constitute one of the main causal agents. Enteroviruses are one of the most viruses monitored in water and are often used as an indicator of viral pollution. Isolation and identification of this virus are now regularly based on molecular tools. However published or commercial protocols for detection of these viruses in water are frequently lacking of validation processes and performance evaluation in such complex samples. A method for enterovirus detection in environmental water has been developed, its performance has been evaluated and compared with several commercial kits. The sensitivity of commercial methods in clinical samples, ranged between 89% and 100%, while the sensitivity in seeded environmental matrices fell between 16% and 91%. This method showed the best performance in environmental samples and was subsequently applied on surface and treated wastewater. The results showed the large dissemination of enteroviruses in an urbanized river. The results also emphasized the importance of good knowledge of the method's limits for its utilization in environmental samples in order to minimize false negatives and to avoid underestimating viral concentration. (C) 2014 Elsevier B.V. All rights reserved.