期刊
JOURNAL OF VIROLOGICAL METHODS
卷 204, 期 -, 页码 101-104出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2014.03.028
关键词
Rapid diagnosis; RT-LAMP; H7N9; Avian influenza virus
资金
- Grants-in-Aid for Scientific Research [26860311] Funding Source: KAKEN
A genetic diagnosis system for detecting avian influenza A (H7N9) virus infection using reverse transcription-loop-mediated isothermal amplification (RT-LAMP) technology was developed. The RT-LAMP assay showed no cross-reactivity with seasonal influenza A (H3N2 and H1N1pdm09) or influenza B viruses circulating in humans or with avian influenza A (H5N1) viruses. The sensitivity of the RT-LAMP assay was 42.47 copies/reaction. Considering the high specificity and sensitivity of the assay for detecting the avian influenza A (H7N9) virus and that the reaction was completed within 30 mm, the RT-LAMP assay developed in this study is a promising rapid diagnostic tool for avian influenza A (H7N9) virus infection. (c) 2014 Elsevier B.V. All rights reserved.
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