期刊
JOURNAL OF VIROLOGICAL METHODS
卷 189, 期 2, 页码 379-382出版社
ELSEVIER
DOI: 10.1016/j.jviromet.2013.02.008
关键词
Lateral-flow immunoassay; Fluorescence; Enhanced influenza sensitivity
Sofia, a novel, fluorescent lateral-flow immunoassay was compared with two conventional calorimetric assays, Quickvue Influenza A+B and Directigen FLU A+B, to identify influenza viral antigen from patient nasopharyngeal specimens. A total of 118 frozen original influenza-positive specimens and 57 prospective specimens were examined. Using rt-PCR as a referee assay, sensitivity values (%) for influenza A/B of 80.0/74.8,73.3/59.3 and 73.3/40.7 were obtained using the Sofia, Quickvue and Directigen assays, respectively. All assays demonstrated reduced sensitivity for influenza B as compared with influenza A virus. With respect to the Sofia assay, the sensitivity of influenza B for the Directigen assay was significantly diminished. False positive results were not observed in the Sofia and Directigen assays. The Quickvue assay produced 3 false-positive results (2 influenza A and 1 influenza B) resulting in a specificity (%) of 96 and 98 for influenza A and B, respectively. Cross-reactivity to other respiratory viruses was not observed among immunoassays. A sensitivity rank (highest to low) of rt-PCR > culture > Sofia> Quickvue > Directigen was established using dilutions of influenza A and B. Sofia provides enhanced sensitivity and objective result interpretation over conventional colorimetric immunoassays. (c) 2013 Elsevier B.V. All rights reserved.
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