期刊
JOURNAL OF VIROLOGICAL METHODS
卷 189, 期 1, 页码 36-40出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2013.01.002
关键词
Calves; Diarrhea; Bovine rotavirus; Genotype; Real-time PCR
资金
- Science and Technology Program project in Gansu province of China [0708NKCA07]
- Research and Application Project of Agricultural Biotechnology in Gansu province [GNSW-2010-10]
To investigate the epidemical characteristics and genotype distributions of bovine rotavirus (BRV) in China, 195 fecal samples were collected from calves with diarrhea in China. Fecal samples were detected for rotavirus A antigen using ELISA. The positive samples were screened for VP7 and VP4 by RT-PCR. G serotyping and P genotyping were conducted on 53 VP7 and VP4 positive samples using RT-PCR. The results showed that 82 samples were found positive for BRV. 752 bp, 660 bp and 285 bp bands were amplified for G-typing. 478 bp, 375 bp and 361 bp bands were amplified for P-typing. The G6 and G10 serotypes were 29(54.7%) and 8 (15.1%) in positive samples for VP7. P[5] and P[11] genotypes were 28 (52.8%) and 10 (18.9%) in the positive samples for VP4. The main combinations of BRV G serotype and P genotype were G6P[5] (28.3%), G6P[5]P[1] (5.7%), GlOP[5] (5.7%) and GlOG6P[5] (5.7%), respectively. Other combinations (including untypable) of G serotype and P genotype were 54.6%. The dominant G serotype and P genotype were G6 and P[5] respectively. The predominant combination of G and P serotypes was G6P[5]. This has significance for establishing the preventive measures against diarrhea caused by group A rotaviruses in cattle. (C) 2013 Elsevier B.V. All rights reserved.
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