期刊
JOURNAL OF VIROLOGICAL METHODS
卷 180, 期 1-2, 页码 68-74出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2011.12.013
关键词
Rift Valley fever virus; Monoclonal antibody; Antigen-capture ELISA
资金
- Ministry of Health, Labour, and Welfare of Japan [H19-Shinkou-Ippan-012, H19-Shinkou-Ippan-003, H22-Shinkou-Ippan-006]
- Grants-in-Aid for Scientific Research [21406012] Funding Source: KAKEN
Monoclonal antibodies (MAbs) raised against the nucleoprotein (NP) of Rift Valley fever virus (RVFV) were developed, and an antigen-capture enzyme-linked immunosorbent assay (Ag-capture ELISA) system was developed for the detection of RVFV NP. The assay detected RVFV antigen from culture supernatants containing as little as 7.8-31.3 pfu per 100 mu l Reactivity with various truncated NPs indicated that MAb C10-54 bound only to the full-length NP, probably due to recognition of a conformational epitope, whereas MAbs G2-36 and D5-59 bound to a linear epitope ranging from amino acid residues 195-201 in the C-terminal region. Based on the alignments of the amino acid sequence of RVFV NP, the epitope regions of MAbs G2-36 and D5-59 were completely conserved among all RVFV strains. These results suggest that the MAbs are applicable to the Ag-capture ELISA for the diagnosis of RVFV infections. (C) 2011 Elsevier B.V. All rights reserved.
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