期刊
JOURNAL OF VIROLOGICAL METHODS
卷 171, 期 1, 页码 310-313出版社
ELSEVIER
DOI: 10.1016/j.jviromet.2010.11.010
关键词
Filovirus; Marburg virus; Ebola virus; Diagnosis; RT-PCR
资金
- Ministry of Health, Labor and Welfare of Japan
- Founding Research Centers for Emerging and Reemerging Infectious Diseases
- Ministry of Education, Culture, Sports, Science, and Technology, Japan
- National Microbiology Laboratory of the Public Health Agency of Canada
- Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institute of Health
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [ZIAAI001089] Funding Source: NIH RePORTER
The filoviruses, Marburg virus (MARV) and Ebola virus (EBOV), are causative agents of severe hemorrhagic fever with high mortality rates in humans and non-human primates. Sporadic outbreaks of filovirus infection have occurred in Central Africa and parts of Asia. Identification of the natural reservoir animals that are unknown yet and epidemiological investigations are current challenges to forestall outbreaks of filovirus diseases. The filovirus species identified currently include one in the MARV group and five in the EBOV group, with large genetic variations found among the species. Therefore, it has been difficult to develop a single sensitive assay to detect all filovirus species, which would advance laboratory diagnosis greatly in endemic areas. In this study, a highly sensitive universal RT-PCR assay targeting the nucleoprotein (NP) gene of filoviruses was developed. The genomic RNAs of all known MARV and EBOV species were detected by using an NP-specific primer set. In addition, this RT-PCR procedure was verified further for its application to detect viral RNAs in tissue samples of animals infected experimentally and blood specimens of infected patients. This assay will be a useful method for diagnostics and epidemiological studies of filovirus infections. (C) 2010 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据