期刊
JOURNAL OF VIROLOGICAL METHODS
卷 171, 期 1, 页码 111-116出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2010.10.010
关键词
Baculovirus; Plaque assay; Virus Counter; Recombinant protein; Virus quantitation
资金
- National Institutes of Health, National Institute of Allergy and Infectious Diseases(NIH/NIAID) [5 R44 AI068270-04]
- National Cancer Institute (NCI) [P30 CA125123-03]
- NATIONAL CANCER INSTITUTE [P30CA125123] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R44AI068270] Funding Source: NIH RePORTER
The utility of a new instrument for rapid virus quantitation, the Virus Counter, was evaluated in a blind study conducted at three sites. This instrument is a substantially improved version of the original academic research instrument described previously by Stoffel and Rowlen (2005a). The addition of hydrodynamic focusing, a self-contained fluidics system and customized software for system control and data analysis has resulted in a commercially viable and available design. Baculovirus samples were provided by Protein Sciences Corporation and blinded to InDevR and Baylor College of Medicine. Protein Sciences Corporation and Baylor College of Medicine analyzed the samples by plaque assay and InDevR analyzed the samples using the Virus Counter. Serial dilution of stock viruses into growth media and buffer allowed for comparison of measured versus intended concentrations. Direct log-scale comparison between pooled Virus Counter results and pooled plaque assay results indicated a linear relationship (slope = 1.1 +/- 0.2, R-2 = 0.86) with statistically significant Pearson correlation (r = 0.93, p < 0.001). (C) 2010 Elsevier B.V. All rights reserved.
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