期刊
JOURNAL OF VIROLOGICAL METHODS
卷 178, 期 1-2, 页码 87-93出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2011.08.018
关键词
West Nile virus (WNV); DNA-based replicon; Vaccine
资金
- National Natural Science Foundation of China [30972613, 31000083]
- Major Special Program of National Science and Technology of China [2009ZX10004-015, 2008ZX10004-015]
While self-replicating, non-infectious subgenomic flavivirus replicons have been described, most of them are RNA transcripts under the control of an Sp6 or T7 promoter. In this study, using West Nile virus (WNV) as a model, a series of DNA-based reporter replicons under the control of a minimal cytomegalovirus (CMV) immediate-early promoter were constructed, and functional analysis showed that these reporter replicons replicate efficiently in mammalian cells. When the DNA-based WNV replicon was used to immunize mice, NS1-specific IgG antibodies and anti-WNV neutralizing antibodies were both induced. Additionally, immunization with this DNA-based WNV replicon induced high levels of lymphocyte proliferation and enhanced the secretion of IFN-gamma. These results suggest that this type of DNA-based replicon can induce humoral and cellular immune responses in mice, indicating that this type of DNA-based replicon may serve as a useful platform for vaccine development and protein expression. (C) 2011 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据