期刊
JOURNAL OF VIROLOGICAL METHODS
卷 171, 期 1, 页码 306-309出版社
ELSEVIER
DOI: 10.1016/j.jviromet.2010.10.028
关键词
Bovine ephemeral fever virus; RT-LAMP; RT-PCR; Virus isolation
资金
- General Foundation of Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences
- [BRF080305]
A novel reverse-transcription, loop-mediated isothermal amplification (RT-LAMP) assay for the detection of bovine ephemeral fever virus (BEFV) was developed and evaluated in this study. The RT-LAMP assay exhibited higher sensitivity when compared with conventional reverse-transcription polymerase chain reaction (RT-PCR) and virus isolation methods. The specificity of the assay was determined by digestion of the RT-LAMP products with restriction enzyme and detection of BEFV serogroup rabies virus (RV). Using RT-LAMP, RT-PCR and virus isolation methods, 36 blood samples were tested and the results indicated that RT-LAMP could detect early infection with BEFV. The RT-LAMP method is useful for the diagnosis of BEFV infection in blood samples. (C) 2010 Published by Elsevier B.V.
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