期刊
JOURNAL OF VIROLOGICAL METHODS
卷 165, 期 2, 页码 139-144出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2010.01.001
关键词
Foot-and-mouth disease; ELISA; Immunochromatographic strip
资金
- National Natural Science Foundation of China [30730068]
- Chinese National High technology Research and Development Program [2007AA100606]
An immunochromatographic strip was developed for the serological detection of type 0 foot-and-mouth disease (FMD) in swine. In the strip, the expressed protein of VP1, the main protective antigen of FMDV, labeled with colloidal gold was used as the detector, the staphylococcal protein A (SPA) and swine anti-foot-and-mouth disease virus (FMDV) antibody were blotted on the nitrocellulose membrane for the test and control lines, respectively. 296 swine serum samples were collected to evaluate the characteristics of the strip in comparison with existing commercial liquid-phage blocking ELISA (LPB ELISA) kit and peptide ELISA kit. The strip was shown to be of high specificity and sensitivity. Furthermore, the dipstick assay based on the strip is rapid (5 min) and easy to perform with no requirement of professional skills, reagents nor equipment. This suggests that the immunochromatographic strip is an acceptable alternative for use in clinical laboratories lacking specialized equipment and for field diagnosis. (C) 2010 Elsevier B.V. All rights reserved.
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