期刊
JOURNAL OF VIROLOGICAL METHODS
卷 164, 期 1-2, 页码 135-138出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2009.11.031
关键词
Proviral burden; Real-time PCR; Marker; Sequence
资金
- Italian Ministry of Health [30G.27]
The use of HIV-1 DNA quantitation in cellular reservoirs to predict disease progression and treatment outcome in infected patients is hampered by the lack of standardization among the available methods. In the present study, real-time PCR methods used commonly for HIV-1 proviral DNA evaluation were compared, showing strong differences in the results, probably as a consequence of genome variability in the target regions. Standardization of HIV-1 proviral DNA quantitation assays is needed for use in clinical management of patients with HIV-1. (C) 2010 Published by Elsevier B.V.
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