期刊
JOURNAL OF VIROLOGICAL METHODS
卷 160, 期 1-2, 页码 69-73出版社
ELSEVIER
DOI: 10.1016/j.jviromet.2009.04.019
关键词
CSFV; C-strain vaccine; PriProET; Real-time PCR
资金
- EU
- Network of Excellence for Epizootic Disease Diagnosis and Control
Classical swine fever (CSF) is a contagious and devastating disease, causing serious losses in the pig industry worldwide. Vaccination of pigs with the conventional C-strain vaccine has been practised in different regions of the world in order to prevent the disease. In the control programmes of CSF, rapid detection and identification of the causing agent, classical swine fever virus (CSFV) is a crucial step. This study describes a novel real-time PCR assay based on primer-probe energy transfer (PriProET) technology for improved detection of CSFV. The assay is able to detect 20 copies of viral cDNA per reaction, showing a high sensitivity. The specificity has been evaluated by testing 57 pestiviruses, representing all species and unclassified pestiviruses. The assay has been found to be highly reproducible. Following PCR amplification, melting curve analysis allows confirmation of specific amplicons, and differentiation between wild-type CSFV and certain C-strain vaccines. This study provides a new tool for the diagnosis of CSF. (C) 2009 Elsevier B.V. All rights reserved.
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