期刊
JOURNAL OF VIROLOGICAL METHODS
卷 154, 期 1-2, 页码 223-225出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2008.07.004
关键词
HHV-6; Variant; LAMP; Genotyping
资金
- grant-in-aid for the Open Research Center
- Ministry of Education, Culture, Sports, Science and Technology of Japan
- Ministry of Health, Labour and Welfare of Japan [H18-Shinko-004]
Genotyping of human herpesvirus 6 (HHV-6) is important clinically, particularly for the diagnosis of neurological diseases. The objective of this study was to establish a rapid HHV-6 genotyping method using the loop-mediated isothermal amplification (LAMP) method. An Accl site is located in the target sequence of HHV-6 B, but not in HHV-6 A. LAMP products were digested with the Accl enzyme and then separated by agarose gel electrophoresis to differentiate the digest pattern of the two variants. The fragment patterns were clearly different between HHV-6 A and B. In order to evaluate the reliability of this HHV-6 genotyping method for use in the clinical laboratory, serum samples from 20 patients with either primary HHV-6 infection or viral reactivation were collected and analyzed. HHV-6 DNA was amplified directly from the serum samples and all 20 LAMP products were positive for HHV-6 B. (C) 2008 Elsevier B.V. All rights reserved.
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