期刊
JOURNAL OF VIROLOGICAL METHODS
卷 153, 期 2, 页码 238-240出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2008.08.004
关键词
Rotavirus; Gastroenteritis; Quantitative RT-PCR
The standard diagnosis of rotavirus gastroenteritis is based on the demonstration of rotavirus antigen in stools using an enzyme immunoassay (EIA). In this study, a one-step quantitative RT-PCR (Q-PCR) was used for sensitive detection of rotavirus in diarrheal stools. The primers and TaqMan probe for the Q-PCR were selected from a highly conserved region of the non-structural protein 3 (NSP3) of rotavirus. After validation, the test was applied to study rotavirus EIA positive (N = 25) and EIA negative (N = 143) stool specimens from cases of acute gastroenteritis of all degrees of severity in a prospective follow-up cohort of infants from 2 months to 2 years of age. Q-PCR detected all 25 EIA positive rotavirus antigens and seven additional cases that were rotavirus EIA negative, i.e. 28% more rotavirus positive cases than identified by EIA. It is concluded that Q-PCR using primers targeted at NSP3 is a rapid and sensitive method for diagnosing acute rotavirus gastroenteritis. (C) 2008 Elsevier B.V. All rights reserved.
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